Using Rhizosphere Phosphate Solubilizing Bacteria to Improve Barley (Hordeum vulgare) Plant Productivity.

On average less than 1% of the total phosphorous present in soils is available to plants, making phosphorous one of the most limiting macronutrients for crop productivity worldwide. The aim of this work was to isolate and select phosphate solubilizing bacteria (PSB) from the barley rhizosphere, which has other growth promoting traits and can increase crop productivity. A total of 104 different bacterial isolates were extracted from the barley plant rhizosphere. In this case, 64 strains were able to solubilize phosphate in agar plates. The 24 strains exhibiting the highest solubilizing index belonged to 16 different species, of which 7 isolates were discarded since they were identified as putative phytopathogens. The remaining nine strains were tested for their ability to solubilize phosphate in liquid medium and in pot trials performed in a greenhouse. Several of the isolated strains (Advenella mimigardefordensis, Bacillus cereus, Bacillus megaterium and Burkholderia fungorum) were able to significantly improve levels of assimilated phosphate, dry weight of ears and total starch accumulated on ears compared to non-inoculated plants. Since these strains were able to increase the growth and productivity of barley crops, they could be potentially used as microbial inoculants (biofertilizers).

The Impact of an Authentic Sports Leadership Program for Coach.

Purpose: This paper studies the effects of authentic sports leadership training on coaches' self-perception of their own authentic leadership, perceived justice, competence, overall self-efficacy, and collective efficacy. Additionally, players' perceptions of their coaches with respect to their authentic leadership, perceived justice, competence, collective efficacy, satisfaction with the coach, and support for basic psychological needs were analyzed. Design: Twenty-five football and handball coaches were randomly assigned to two groups. Fifteen coaches made up the experimental group that carried out the training leadership program, while 10 coaches made up the control group, carrying out no training whatsoever. A total of 248 football and handball players participated in this study; 136 were led by coaches who participated in the training program, and 112 by coaches who did not participate in the program. Results: The results of this study indicate that coaches' self-perception is positively influenced after having received training in the variables of authentic leadership, perceived justice, competence, overall self-efficacy, and collective efficacy. Players whose coaches were part of the program perceive them as being more competent as coaches. Conclusions: The effects of an authentic sports leadership training program are effective for coaches and players alike.

Milk Quality and Carbon Footprint Indicators of Dairy Sheep Farms Depend on Grazing Level and Identify the Different Management Systems.

Currently, there are very few studies in the dairy sheep sector associating milk quality and indicators regarding carbon footprint and their link to grazing levels. For 1 year, monthly milk samples and records related to environmental emissions and management systems were collected through surveys from 17 dairy sheep farms in the region of Castilla y León (Spain), in order to relate this information to the use of natural pastures under free grazing. Indicators were constructed on the collected data and subjected to a multivariate statistical procedure that involved a factor analysis, a cluster analysis and a population canonical analysis. By applying multivariate statistical techniques on milk quality and carbon footprint indicators, it was possible to identify the management system of the farms. From an environmental point of view, farms with a higher grazing level (cluster 4) were more sustainable, as they had the lowest carbon footprint (lower CO2, N2O and CO2 equivalent emissions per sheep and year) and the lowest energy consumption levels, which were gradually lower than those of farms in cluster 3; both indicators were much lower than those of farms in clusters 1 and 2. The milk quality of cluster 1 and 2 farms was significantly lower in terms of total protein and fat content, dry extract, omega-3 fatty acid levels and α-tocopherol content than farms in clusters 3 and 4, which had higher accessibility to grazing resources. In sum, the higher the use of natural resources, the lower the external inputs the farms required and the lower environmental impact and energy costs they have.

Evaluating the effect of biochar addition on the anaerobic digestion of swine manure: application of Py-GC/MS.

The anaerobic digestion process of swine manure was studied when char was used as supplement for improving performance. The use of pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) was proposed for assessing the organic matter degradation. The assessment on biogas production was carried out using samples of swine manure (SM) supplemented with char in one case and pre-treated by microwave irradiation in the other. This experimental set-up allows for the comparison of the biological degradation observed under these two different configurations and therefore aids in understanding the effect of char particles on the process. Results showed similar performance for both systems, with an average improvement of 39% being obtained in methane production when compared to the single digestion of SM. The analysis of digestate samples by Fourier transform infrared (FTIR) spectroscopy and Py-GC/MS showed improved degradation of proteins, with the Py-GC/MS technique also capable of identifying an increase in microbial-derived material when char was added, therefore highlighting the relevant role of carbon conductive particles on biological systems. Py-GC/MS along with the use of FTIR spectroscopy has proven to be useful tools when evaluating anaerobic digestion.

Relationships between Sprint Ability and Endurance Capacity in Soccer Referees.

The aim of this study was to analyze the association between sprint ability and endurance capacity in soccer referees. Twenty-three Spanish officials participated in this study. Each referee undertook, in this order, a 40 m linear straight sprinting test (40 m Sprint) and the Yo⁻Yo intermittent recovery level 1 test (YYIR1) interspersed with a 8 min of self-administered rest. The results in the 40 m Sprint test showed that the time spent by referees was 5.56 ± 0.27 s and achieved a maximum velocity of 31.46 ± 2.85 km·h-1. Furthermore, during the YYIR1 the referees covered 1213.91 ± 432.26 m. The distance covered at YYIR1 was moderately correlated to the velocity achieved in the 40 m Sprint test (r = -0.404, p < 0.05). These results suggest that the ability to reach high speeds is a limiting factor in YYIR1 performance.

Effect of Anatase Synthesis on the Performance of Dye-Sensitized Solar Cells.

Anatase nanoparticles were synthesized from a titanium isopropoxide solution using a hydrothermal process at different pressures in an autoclave system while keeping the volume of the solution constant. As the autoclave pressure was increased from 1 to 71 atm (23 to 210 °C), the crystal size in the nanoparticles increased from 9 to 13.8 nm. The anatase nanoparticles were used to build dye-sensitized solar cells (DSSC). Mesoporous films of this oxide were deposited over conducting SnO2:F substrates using the screen-printing technique and then annealed at 530 °C at 1 atm of air pressure. The morphology of the mesoporous film surface of anatase, studied using scanning electron microscopy, revealed that the crystal size and pore distribution were functions of the pressure conditions. The energy band gap of the films as a function of the crystal size exhibited quantum effects below 11.8 nm. The effects of the anatase synthesis conditions and properties of the mesoporous film on the DSSC-type solar cell parameters, η%, V OC, J SC, and FF, were also investigated: the mesoporous anatase films prepared at 200 °C (54 atm of pressure in the autoclave) and annealed at 530 °C in air generated the best solar cell, having the highest conversion efficiency.